Zobrazení zdroje stránky Johanssonkendall7190

The development of a safe, effective and affordable prophylactic vaccine against hepatitis C virus (HCV) remains a medical priority. Hepatitis B-C subviral envelope particles, which could be produced by industrial procedures adapted from those established for the hepatitis B virus vaccine, appear promising for use for this purpose. The prototype HBV-HCV bivalent vaccine-bearing genotype 1a HCV envelopes can induce neutralizing antibodies against this genotype, but is less effective against other genotypes. We show here, in a small animal model, that the use of a set of vaccine particles harbouring envelopes from different HCV genotypes in various association strategies can induce broad neutralizing protection or an optimized protection against a particular genotype prevalent in a given region, such as genotype 4 in Egypt. This vaccine could help to control the hepatitis C epidemic worldwide.The aim of the randomised trial was to compare conventional semen parameters, sperm DNA fragmentation levels and satisfaction levels between semen samples collected at home and at the clinic. We recruited 110 men with a history of infertility for at least 1 year from the outpatient andrology clinic. Each man collected two semen samples, one at home and one at the clinic. Men were randomly assigned into the home first (n = 55) or clinic first (n = 55) groups. The primary outcome was sperm concentration. There was no significant difference in sperm concentration, sperm DNA fragmentation levels or other conventional semen parameters between home first and clinic first samples (p &gt; .05), while satisfaction levels were significantly higher for home first samples (p less then .01). Consistent results were obtained when comparing home-collected and clinic-collected samples within individuals. Men can be offered the option to collect semen samples at home for examination or assisted reproduction without compromising semen quality, especially for those with difficulty in producing semen samples at the clinic.Two domestic shorthair cats were presented with anorexia and dehydration following ingestion of caramelized onions. Shared key findings from a CBC (ADVIA 2120), serum biochemistry, and urinalysis included a spurious, marked leukocytosis with discordant basophil (BASO) channel and peroxidase channel WBC counts, normal manual leukocyte counts, mild, non-regenerative anemia with discrepancies between automated and manual reticulocyte counts, an abundance of large Heinz bodies (HBs), and highly irregular scattergrams. Case 1 also demonstrated a markedly elevated mean corpuscular hemoglobin concentration (MCHC) and discrepancies between RBC hemoglobin indices. Spurious leukocyte results were confirmed through re-analysis of samples (including the acquisition of a new sample, use of an alternate analyzer (Sysmex XT-2000iV; Case 1 only), and evaluation of scattergrams and blood films (Cases 1 and 2). Repeatedly discrepant reticulocyte counts were also identified. In both cases, the erroneous BASO WBC counts, discrepancies in reticulocyte counts and RBC indices, and atypical scattergrams were interpreted to result from various effects of the HBs. These cases emphasize the importance of reviewing blood films, interpreting scattergrams, and the usefulness of duplicate methods for determining various measurands on hematology analyzers.Oncocytic cell tumor of the thyroid is composed of large polygonal cells with eosinophilic cytoplasm that is rich in mitochondria. These tumors frequently have the mutations in mitochondrial DNA encoding the mitochondrial electron transport system complex I. However, the mechanism for accumulation of abnormal mitochondria is unknown. A non-canonical mitophagy system has recently been identified, and mitochondria-eating protein (MIEAP) plays a key role in this system. We therefore hypothesized that accumulation of abnormal mitochondria could be attributed to defective MIEAP expression in these tumors. We first show that MIEAP was expressed in all the conventional thyroid follicular adenomas (FAs)/adenomatous goiters (AGs) but not in oncocytic FAs/AGs, while its expression was defective not only oncocytic thyroid cancers but also in the majority of conventional thyroid cancers. MIEAP expression was not correlated with methylation status of 5'-untranslated region of the gene. Our functional analysis demonstrated that exogenously induced MIEAP but not PARK2 reduced the amounts of abnormal mitochondria, as demonstrated by decreased reactive oxygen species levels, mitochondrial DNA/nuclear DNA ratios and cytoplasmic acidification. Therefore, together with previous studies showing that impaired mitochondrial function triggers compensatory mitochondrial biogenesis that causes an increase in the amounts of mitochondria, we would like to conclude that, in oncocytic cell tumors of the thyroid, increased abnormal mitochondria cannot be efficiently eliminated because of a loss of MIEAP expression, i.e., impaired MIEAP-mediated non-canonical mitophagy.Introduction and aims Smokers differ in how they smoke and how they stop smoking. Use of nicotine replacement therapy (NRT) and e-cigarettes further diversifies smokers. We aimed to identify and compare latent groups of past-year smokers and to describe longitudinal transitions between the identified groups. Design and methods Latent transition analysis of online UK past-year smokers' data (n = 2857) collected in June 2016 and followed-up in October 2017. Latent groups were identified based on participants' smoking, e-cigarette use, NRT use, urges to smoke and last quit attempt. Sociodemographic and smoking characteristics between the groups were compared using Pearson's χ 2 test and Cramer's V. Results Four latent groups of smokers (heavy smokers, light smokers, smokers using NRT, smokers using e-cigarettes) and two recently quit smokers (abstinent ex-smokers, ex-smokers using e-cigarettes) were identified. Nearly half the participants (48.9%) were heavy or light smokers who did not use alternative nicotine products and largely had not tried quitting smoking. [https://www.selleckchem.com/CDK.html CDK and cancer] Latent groups were relatively stable and transitions to quitting smoking were more probable in groups of smokers using alternative nicotine products. Smokers using NRT transitioned mostly to abstinent ex-smokers, and smokers using e-cigarettes to ex-smokers using e-cigarettes' groups (11.2% and 11.4%, respectively). The abstinent ex-smokers group grew the most at follow-up (+6.3%). Discussion and conclusions Nearly half of the participants continued smoking without having tried to reduce harm or quit. Of smokers who transitioned, the majority quit smoking and abstained from nicotine altogether. Use of alternative nicotine products alongside smoking was associated with higher probabilities of becoming an ex-smoker.