Zobrazení zdroje stránky Hoffmandalsgaard9826

Whole-wheat flour (WWF) is increasingly popular because of the health benefits of whole grains. This study investigated the effect of WWF particle size on dough properties, bread quality and in vitro starch digestibility. WWF was made from intact whole grain directly. Three WWF particle sizes were examined, including coarse, medium and fine with a mean size of 1315, 450 and 199 μm, respectively. The dough made from WWF of a larger particle size exhibited lower extensibility and stability, and subsequently the bread had a more compact structure (i.e. lower open porosity and thicker cell thickness), smaller specific volume and harder texture, which were regarded as poor quality attributes. On the other hand, the bread made from the fine WWF exhibited a higher amount of released glucose than those made from the coarse and medium WWFs. Moreover, the particle size of bread bolus showed no significant effect on in vitro starch digestion. The whole study demonstrated that the particle size of WWF plays a critical role in determining both bread quality and digestibility.We propose a novel class of gold-containing molecules, which have been designed using conjugated carbon structures as templates. The sp-hybridized carbons of C2 moieties are replaced with a gold atom and one of the adjacent carbons is replaced by nitrogen. Applying the procedure to hexadehydro[12]annulene yields the well-known cyclic trinuclear gold(i) carbeniate complex. Planar, tubular and cage-shaped complexes can be obtained by taking similar sp-hybridized carbon structures as the starting point.A liquid crystal biosensor based on DNA aptamer for sensitive detection of Parkinson's Disease (PD) related alpha-synuclein was developed. This LC biosensor is constructed using a simple and label free method, and it not only enables early PD diagnosis, but also provides a general platform for detection based on DNA aptamer.Grease, as used for lubrication of rolling bearings, is a two-phase organogel that slowly releases oil from its gelator matrix. Because the rate of release determines the operation time of the bearing, we study this release process by measuring the amount of extracted oil as a function of time, while we use absorbing paper to speed up the process. The oil concentration in the resulting stain is determined by measuring the attenuation of light transmitted through the paper, using a modified Lambert-Beer law. For grease, the timescale for paper imbibition is typically 2 orders of magnitude larger than for a bare drop of the same base oil. This difference results from the high affinity, i.e. wetting energy per unit volume, of the oil for the grease matrix. To quantify this affinity, we developed a Washburn-like model describing the oil flow from the porous grease into the paper pores. The stain radius versus time curves for greases at various levels of oil content collapse onto a single master curve, which allows us to extract a characteristic spreading time and the corresponding oil-matrix affinity. Lowering the oil content results in a small increase of the oil-matrix affinity yet also in a significant change in the spreading timescale. Even an affinity increase of a few per mill doubles the timescale.The Ru(ii) complex of an imidazole-mesalazine Schiff base is a unique example showing growth inhibition of 3D-colon cancer stem cell spheroids and bulk colon cancer cells at lower dosage than salinomycin or oxaliplatin. Unlike oxaliplatin which increases the expression of stemness genes (SOX2, KLF4, HES1 and Oct4), these complexes maintain a tight regulation.The structural features of the matrix surrounding the cells play a crucial role in regulating their behavior. Here, we used fluorescence microscopy and customized analysis algorithms to characterize the architecture of fibrous hydrogel networks. As a model system, we investigated a new class of synthetic biomimetic material, hydrogels prepared from polyisocyanides. Our results show that these synthetic gels present a highly heterogeneous fibrous network, with pores reaching a few micrometers in diameter. By encapsulating HeLa cells in different hydrogels, we show that a more porous structure is linked to a higher proliferation rate. The approach described here, for the characterization of the network of fibrous hydrogels, can be easily applied to other polymer-based materials and provide new insights into the influence of structural features in cell behavior. This knowledge is crucial to develop the next generation of biomimetic materials for 3D cell models and tissue engineering applications.Many volatile organic compounds (VOCs) are hazardous to human health and their concentrations need to be monitored over a large area. However, most chemical sensors can only be used for point detection. In this paper, we report a method to obtain a two-dimensional (2D) concentration profile of toluene vapor by using polymer-stabilized liquid crystals (PSLCs). After the PSLC sample is exposed to toluene vapor ranging from 9300 to 2800 ppm, the PSLC changes its interference color according to different local toluene concentrations. By using the interference color, we can obtain a 2D concentration profile of toluene inside the PSLC and study the diffusion of toluene inside the PSLC in great detail. We also determine the diffusion coefficient of dissolved toluene inside the PSLC to be 1.01 × 10-6 cm2 s-1.Lithium-ion batteries have had a tremendous impact on several sectors of our society; however, the intrinsic limitations of Li-ion chemistry limits their ability to meet the increasing demands of developing more advanced portable electronics, electric vehicles, and grid-scale energy storage systems. Therefore, battery chemistries beyond Li ions are being intensively investigated and need urgent breakthroughs toward commercial applications, wherein the use of metallic Li is one of the most intuitive choices. Despite several decades of oblivion due to safety concerns regarding the growth of Li dendrites, Li-metal anodes are now poised to be revived because of the advances in investigative tools and globally invested efforts. [https://www.selleckchem.com/PARP.html PARP signaling] In this review, we first summarize the existing issues with regard to Li anodes and their underlying reasons and then highlight the recent progress made in the development of high-performance Li anodes. Finally, we propose the persisting challenges and opportunities toward the exploration of practical Li-metal anodes.Catalytic conversion of CO to CO2 has been investigated in ultrahigh vacuum (UHV) under cryogenic conditions (10 K). This cryogenic oxidation is assisted by iron upon its co-deposition with CO, on a substrate. The study shows that the interaction of Fe and CO results in a Fe-CO complex that reacts in the presence of excess CO at cryogenic conditions leading to CO2. Here, the presence of CO on the surface is a prerequisite for the reaction to occur. Different control experiments confirm that the reaction takes place in the condensed phase and not in the gas phase. Surface sensitive reflection absorption infrared spectroscopy (RAIRS), temperature programmed desorption (TPD), and Cs+ based low energy ion scattering are utilized for this study. The iron assisted formation of CO2 may be proposed as another pathway relevant in interstellar ices, containing CO. This direct oxidation process, which occurs at extremely low temperatures and pressures, in the presence of a reactive metal species like iron (the most abundant metal in the interstellar medium) may have astrochemical importance. It does not require any external energy in the form of photo-irradiation or thermal processing. Such reactions are highly relevant in cold dense molecular clouds where interactions between neutral species are more favoured.A highly conjugated polymeric porphyrin with an ethynyl group is proposed as a new cathode for lithium organic batteries. The electrochemical performance is significantly improved after a simple coupled polymerization, resulting in excellent cycling stability with a capacity retention of 99.2% for 2000 cycles.Signal Amplification by Reversible Exchange (SABRE) uses para-enriched dihydrogen, pH2, to boost the NMR signal by several orders of magnitude. Although the method was discovered more than a decade ago, the quest to optimize SABRE and to establish a complete description in silico is ever ongoing. The simulation of SABRE is exacerbated by a complex interplay of chemical exchange and coherent polarization transfer. Here, we compare two different simulation approaches, Markov chain Monte Carlo (MC) simulations and a modification of the Liouville von Neumann equation with superoperators of chemical exchange (SO). We apply these methods to simulate the fate of truncated and full, three and four spin-½ systems in two different experiments continuous polarization at a constant or alternating B0 field. For all cases, MC and SO provided similar results, and previously reported experimental data were well reproduced. It appears that both methods are well suited to approach this matter, while SO was faster than MC by several orders of magnitude in the cases discussed.This study was conducted to investigate behavioral changes in laying hens (Hy-Line Brown) after transfer to a multi-tier system from the floor system and to examine their production performance. The hens were randomly divided into two groups and were allocated to the multi-tier system and the floor system at a commercial farm. Behavior of the laying hens was recorded by CCD (charge-coupled device) cameras and a digital video recorder. The data were scanned every 2 min to obtain an instantaneous behavioral sample or were immediately counted whenever the hens exhibited a designated behavior. Behavior changed dramatically during the first seven days. Egg production was higher in the multi-tier system, while cracked and dirty eggs were more frequent in the floor system (p less then 0.05). No differences in mortality rate or egg quality were observed between the groups. In conclusion, the hens needed at least seven days to adapt to the multi-tier system. The multi-tier system was more efficient than the floor system in terms of production performance. © Copyright 2020 Korean Society of Animal Science and Technology.Studies on promoting milk protein yield by supplementation of amino acids have been globally conducted. Nevertheless, there is a lack of knowledge of what pathways affected by individual amino acid in mammary epithelial cells that produce milk in practice. Phenylalanine (PHE) and valine (VAL) are essential amino acids for dairy cows, however, researches on mammary cell levels are still lacking. Thus, the aim of this study was conducted to evaluate the effects of PHE and VAL on milk protein synthesis-related and energy-mediated cellular signaling in vitro using immortalized bovine mammary epithelial (MAC-T) cells. To investigate the effects of PHE and VAL, the following concentrations were added to treatment medium 0, 0.3, 0.6, 0.9, 1.2, and 1.5 mM. The addition of PHE or VAL did not adversely affect cell viability compared to control group. The concentrations of cultured medium reached its maximum at 0.9 mM PHE and 0.6 mM VAL (p less then 0.05). Therefore, aforementioned 2 treatments were analyzed for proteomics.