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To evaluate the microbiota profile of residual alveolar slits and teeth adjacent to the cleft in fissured individuals.<br /><br /> This study used a cross-sectional design.<br /><br /> Twenty individuals, aged 14 to 24 years, who had a residual fissure in the maxillary alveolar ridge region were selected.<br /><br /> Three sites per individual were selected for microbiological collection (the site of the residual cleft and the 2 nearest teeth). [https://www.selleckchem.com/products/pf-573228.html https://www.selleckchem.com/products/pf-573228.html] The samples were analyzed using the Checkerboard DNA-DNA hybridization technique for 73 species of bacteria.<br /><br /> All the species analyzed were found in the 2 niches (slits and teeth). The bacterial species present in the largest number in the residual cracks were <br /><br /> , <br /><br /> , and <br /><br /> . With regard to the bacterial profiles in the mesial and distal faces, the most prevalent species were <br /><br /> , <br /><br /> , and <br /><br /> . The analysis of all the collected samples demonstrated very similar profiles for the mesial and distal faces, with these 2 sites even presenting the same species in greater frequencies. Higher counts of 20 bacterial species (Wilcoxon test) were observed in the dental niche, in relation to the fissure, particularly, <br /><br /> , <br /><br /> , <br /><br /> , and <br /><br /> .<br /><br /> Some species were significantly more prevalent in the residual alveolar fissures and in adjacent teeth. The comparison between the profiles of the 2 niches demonstrated large differences in the most frequent species in the teeth, and no qualitative differences with regard to specific pathogens.<br /><br />Some species were significantly more prevalent in the residual alveolar fissures and in adjacent teeth. The comparison between the profiles of the 2 niches demonstrated large differences in the most frequent species in the teeth, and no qualitative differences with regard to specific pathogens.Parents who were abused as children are at increased risk for perpetuating maladaptive parenting practices, yet the mechanisms underlying this relationship remain unclear. This study prospectively examined maternal distress (a latent variable consisting of depressive symptoms and daily stress) and family violence as potential mediators in the intergenerational transmission of abusive (i.e., psychologically aggressive and physically assaultive) parenting. Participants included (N = 768) mother-child dyads identified as being at-risk for family violence and maltreatment prior to children's age four. More maternal childhood abuse was associated with more distress and increased risk for family violence exposure in adulthood. However, only maternal distress mediated the association between mothers' history of abuse and their use of abusive parenting strategies. This study provides critical information about ecological mechanisms underlying the intergenerational transmission of abusive parenting and suggests the importance of targeting depression and stress management among mothers with abuse histories to curtail the cycle of violence.Many foodstuffs are extremely susceptible to contamination with aflatoxins, in which aflatoxin B1 is highly toxic and carcinogenic. Therefore, it is crucial to develop a rapid and effective analytical method for detecting and monitoring aflatoxin B1 in food. Herein, a surface-enhanced Raman spectroscopic (SERS) method combined with QuEChERS (quick, easy, cheap-effective, rugged, safe) sample pretreatment technique was used to detect aflatoxin B1. Sample preparation was optimized into a one-step extraction method using an Au nanoparticle-based solution (Au sol) as the SERS detection substrate. An affordable portable Raman spectrometer was then used for rapid, label-free, quantitative detection of aflatoxin B1 levels in foodstuffs. This method showed a good linear log relationship between the Raman signal intensity of aflatoxin B1 in the 1-1000 µg L-1 concentration range with a limit of detection of 0.85 µg kg-1 and a correlation coefficient of 0.9836. Rapid aflatoxin B1 detection times of ∼10 min for wheat, corn, and protein feed powder samples were also achieved. This method has high sensitivity, strong specificity, excellent stability, is simple to use, economical, and is suitable for on-site detection, with good prospects for practical application in the field of food safety.<br /><br /> Prospective lumbar radiograph analysis.<br /><br /> To compare changes in lumbar lordosis in standing flexion versus seated lateral radiographs.<br /><br /> Standing lateral, standing flexion, and seated lateral X-rays of the lumbar spine were obtained in patients presenting with low back pain. Trauma, tumor, and revision cases were excluded. Changes in global lumbar as well as segmental lordosis were measured in each position.<br /><br /> Seventy adult patients were reviewed. Overall, the greatest changes in lordosis were seen at L4-S1 in both the seated and flexion X-rays (12.5° and 6.3°, respectively). Greater kyphosis was seen in seated versus flexion X-rays (21.6° vs 15.8°); changes in lordosis from L1-L3 were similar in both positions, with little change seen at these levels (approximately 5° to 7°). On subgroup analysis, these differences were magnified in analyzing only patients that moved at least 20° globally, and there were no significant differences between sitting and flexion in "stiff" patients that moved less than 20° globally.<br /><br /> Greater lumbar kyphosis was seen in the seated position compared to standing flexion, especially from L4-S1. Given these results we suggest the use of seated lateral X-rays to dynamically assess the lumbar spine. These findings may also guide future research into the mechanism and clinical relevance of a stiff versus mobile lumbar spine, as well as into the sensitivity of seated X-rays in detecting instability.<br /><br />Greater lumbar kyphosis was seen in the seated position compared to standing flexion, especially from L4-S1. Given these results we suggest the use of seated lateral X-rays to dynamically assess the lumbar spine. These findings may also guide future research into the mechanism and clinical relevance of a stiff versus mobile lumbar spine, as well as into the sensitivity of seated X-rays in detecting instability.Haiti has the greatest burden of HIV in the Caribbean. In 2018, the country's HIV prevalence was 2% with an estimated 2200 AIDS-related deaths. Using 2016-2017 Demographic and Health Survey (DHS) data, Haitian men's self-reported ever-having HIV testing was analyzed with a focus on their regions of residence. Only 34% of the men reported ever-having HIV testing. Men who lived in Northern region (aOR1.59, 95%CI1.23-2.05), and Southern region (aOR1.26, 95%CI1.04-1.53) had higher odds of ever-having HIV testing compared to men residing in Central region. Further research should prioritize targeted health promotion for engaging Haitian men who are younger, poorer, with low-level of education and single as well as those who reside in regions where HIV testing is not easily accessible. Haitian men who have not had an HIV test must first be identified and demographic-specific interventions and programming should be used to increase HIV testing among this population.