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To evaluate the applicability of the Mimosa tenuiflora and Eucalyptus urograndis pyroligneous acids (PAs) as alternative antiseptics in dairy goats.<br /><br /> Cytotoxicity was evaluated in vitro using bacteria, as well as in vivo using goats, and the influence of PAs on the physicochemical parameters of fresh milk were examined. The cytotoxicity of PAs was evaluated in terms of morphology, cell viability and metabolic activity of goat tegumentary cells. The PA of M. tenuiflora had results similar to those of 2% iodine. For the in vitro tests, strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used with the well technique, demonstrating inhibition halos greater than 9mm. In the in vivo test, 15 animals were used per phase of the experiment, and the plate counting technique showed that there was antiseptic action of both extracts, with emphasis on the M. tenuiflora PA. Physicochemical analysis of the milk showed that neither PAs interfered with its physical-chemical parameters.<br /><br /> The PA of M. [https://www.selleckchem.com/products/ly3023414.html selleck kinase inhibitor] tenuiflora presented potential as an alternative antiseptic in dairy goats.<br /><br /> This study demonstrates the use of PA as an antimicrobial agent in animals.<br /><br />This study demonstrates the use of PA as an antimicrobial agent in animals.<br /><br /> Safe and efficient nanopesticides for pest control have attracted attention because of their ability to enhance target efficiency and reduce undesirable side effects. Nanoformulations have a significant role in solving the problem of water solubility for insoluble drugs. However, there are few studies on the physicochemical properties and biological activities of pesticides of different particle sizes and remains unclear how these key physicochemical properties are affected by particle size. In this study, a series of glucose-loaded lambda-cyhalothrin nanoparticles (LCNs) with a tunable size were developed via shearing emulsification and carrier loading in order to evaluate insecticidal action.<br /><br /> The mean particle sizes of the LCNs were 50.6, 115.2 and 221 nm. The wettability, dispersibility and stability of nanoparticles were particle size-dependent, and were mainly determined by particle size and the uniformity of distribution. Furthermore, the insecticidal activity of LCNs was inversely proportional to the particle size.<br /><br /> This study not only provides a facile technology for the preparation of nanopesticides with a tunable particle size, but also clarifies the effect of particle size on the performance of pesticides. © 2020 Society of Chemical Industry.<br /><br />This study not only provides a facile technology for the preparation of nanopesticides with a tunable particle size, but also clarifies the effect of particle size on the performance of pesticides. © 2020 Society of Chemical Industry.<br /><br /> Drosophila suzukii (Matsumura, 1931) (spotted wing drosophila), an invasive species, has recently become a significant global pest of soft-skinned fruits such as berries. Unlike other Drosophila species, female D. suzukii have evolved a specialized sharp, serrated ovipositor that pierces and penetrates ripe and ripening fruits, causing them to lose commercial value and preventing their sale. A first step for the development of biological control agents for pest management may be achieved through the identification of microbes infectious for D. suzukii in the wild.<br /><br /> We first determined that D. suzukii is susceptible to chemicals commonly used to rear Drosophilids in the laboratory and established a diet able to sustain healthy D. suzukii growth. Using this diet, we demonstrated that of 25 species of culturable bacteria and fungi isolated from field-collected D. suzukii, eight microbes decreased host survival when injected. Three of the eight bacteria (Alcaligenes faecalis, Achromobacter spanius and Serratia marcescens) were acutely pathogenic to both D. suzukii and Drosophila melanogaster adults by injection. Feeding of these bacteria resulted in susceptibility only in larvae.<br /><br /> We successfully identified multiple microbes from field-collected D. suzukii that are pathogenic to both larvae and adults through different routes of infection, some of which could be candidates for biocontrol of this species. © 2020 Society of Chemical Industry.<br /><br />We successfully identified multiple microbes from field-collected D. suzukii that are pathogenic to both larvae and adults through different routes of infection, some of which could be candidates for biocontrol of this species. © 2020 Society of Chemical Industry.<br /><br /> Workplace gratitude is important for improving work-related outcomes and individual well-being. Although the gratitude at work scale (GAWS) was developed in the United States, it has not been corroborated in Asian countries with interdependent cultures. This study aimed to develop and validate the GAWS among Japanese workers.<br /><br /> Japanese workers completed online surveys at baseline (N=206) and 2weeks later (N=103). The Japanese GAWS was developed according to the international guidelines. We measured (a) trait gratitude as comparison for the criterion-related validity, (b) work-related outcomes/factors (eg, work engagement), and (c) well-being (eg, eudemonic well-being at work) as comparisons for convergent validity. Cronbach's alpha, intra-class correlation coefficients (ICCs), and measurement errors were calculated to assess reliability; measurement validity was evaluated by correlational analyses and confirmatory factor analysis (CFA).<br /><br /> A total of 206 and 93 workers were included for baseline and follow-up analyses, respectively. Cronbach's alpha and ICCs of the Japanese GAWS ranged from 0.81 to 0.91. CFA showed that the 2-factor model (ie, gratitude for (a) a supportive work environment and (b) meaningful work) demonstrated a good fit (χ<br /><br /> (34)=67.58, CFI=0.967, TLI=0.956, RMSEA=0.069, SRMR=0.037), similar to the original version. As we had hypothesized, overall GAWS and the two domains were significantly correlated with trait gratitude, work-related outcomes/factors, and well-being.<br /><br /> The Japanese GAWS demonstrated good reliability and validity. Future research should explore mechanisms related to workplace gratitude and further intervention studies among workers.<br /><br />The Japanese GAWS demonstrated good reliability and validity. Future research should explore mechanisms related to workplace gratitude and further intervention studies among workers.