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The use of aptamers in bioanalytical and biomedical applications exploits their ability to recognize cell surface protein receptors. Targeted therapeutics and theranostics come to mind in this regard. However, protein receptors occur on both cancer and normal cells; as such, aptamers are now taxed with identifying high vs. low levels of protein expression. Inspired by the flexible template mechanism and elegant control of natural nucleic acid-based structures, we report an allosteric regulation strategy for constructing a structure-switching aptamer for enhanced target cell recognition by engineering aptamers with DNA intercalated motifs (i-motifs) responsive to the microenvironment, such as pH. Structure-switching sensitivity can be readily tuned by manipulating i-motif sequences. However, structure-switching sensitivity is difficult to estimate, making it equally difficult to effectively screen modified aptamers with the desired sensitivity. To address this problem, we selected a fluorescent probe capable of detecting G-quadruplex in complicated biological media.Coronavirus disease 2019 (COVID-19) has spread worldwide and become an emerging life-threatening pandemic disease since 2019. This study aimed to evaluate the basic knowledge, awareness, personal hygiene and healthy practices regarding COVID-19 during its outbreak among the Palestinian population in the Gaza Strip. It is a cross-sectional survey carried out between July and August 2020 and included 458 participants, aged 18 years or older, belonging to the five Gaza Strip governorates, Palestine, with a variety of socio-economic status. [https://www.selleckchem.com/products/s-2-hydroxysuccinic-acid.html (S)-2-Hydroxysuccinic acid order] Sociodemographic data and data on COVID-19 knowledge, awareness, personal hygiene and healthy practices were collected via email and social media applications (WhatsApp and Facebook) from the study participants. Statistical analyses were performed using Vr 22 of the SPSS software. Overall, the mean ± SD age of the participants was 26.5 ± 4.2 years. Our findings showed that most of the Gazan adults had very good knowledge regarding COVID-19 in terms of host sources, causative agents, incubation period, symptoms, transmission, treatment and prevention. Additionally, most of the participants had a high level of personal hygiene and healthy practices during the COVID-19 outbreak, where personal hygiene and healthy practices against COVID-19 was 77.4%. Furthermore, a higher educational level did not affect the quality of personal hygiene and healthy practice behaviours during the COVID-19 outbreak. Although most of the Gazans had good knowledge regarding the healthy practices, a significant percentage of the population did not practice the hygiene protocol. Despite the majority of the Gazan population having very good knowledge regarding COVID-19 and a high level of personal hygiene and healthy practices during the COVID-19 outbreak, a small proportion require education about avoiding physical contact and maintaining social distancing with others during the pandemic.In Bangladesh, coronavirus disease 2019 (COVID-19) has been highly prevalent during late 2020, with nearly 500 000 confirmed cases. In the present study, the spike (S) protein of severe acute respiratory coronavirus 2 (SARS-CoV-2) circulating in Bangladesh was genetically investigated to elucidate the diversity of mutations and their prevalence. The nucleotide sequence of the S protein gene was determined for 15 SARS-CoV-2 samples collected from eight divisions in Bangladesh, and analysed for mutations compared with the reference strain (hCoV-19/Wuhan/WIV04/2019). All the SARS-CoV-2 S genes were assigned to B.1 lineage in G clade, and individual S proteins had 1-25 mutations causing amino acid substitution/deletion. A total of 133 mutations were detected in 15 samples, with D614G being present in all the samples; 53 were novel mutations as of January 2021. On the receptor-binding domain, 21 substitutions including ten novel mutations were identified. Other novel mutations were located on the N-terminal domain (S1 subunit) and dispersed sites in the S2 subunit, including two substitutions that remove potential N-glycosylation sites. A P681R substitution adjacent to the furin cleavage site was detected in one sample. All the mutations detected were located on positions that are functionally linked to host transition, antigenic drift, host surface receptor binding or antibody recognition sites, and viral oligomerization interfaces, which presumably related to viral transmission and pathogenic capacity.Clinical descriptions about influenza-like illness in children seem non-specific during the co-circulation of SARS-CoV-2 and influenza. This paper aimed to summarize recent studies comparing clinical features and outcome, laboratory and radiological findings of COVID-19 patients with influenza patients in the paediatric population.Conduction system pacing (CSP) is a technique of pacing that involves implantation of permanent pacing leads along different sites of the cardiac conduction system and includes His bundle pacing and left bundle branch pacing. There is an emerging role for CSP to achieve cardiac resynchronisation in patients with heart failure with reduced ejection fraction and inter-ventricular dyssynchrony. In this article, the authors review these strategies for resynchronisation and the available data on the use of CSP in overcoming dyssynchrony.Cardiac resynchronisation therapy is an important intervention to reduce mortality and morbidity, but even in carefully selected patients approximately 30% fail to improve. This has led to alternative pacing approaches to improve patient outcomes. Left ventricular (LV) endocardial pacing allows pacing at site-specific locations that enable the operator to avoid myocardial scar and target areas of latest activation. Left bundle branch area pacing (LBBAP) provides a more physiological activation pattern and may allow effective cardiac resynchronisation. This article discusses LV endocardial pacing in detail, including the indications, techniques and outcomes. It discusses LBBAP, its potential benefits over His bundle pacing and procedural outcomes. Finally, it concludes with the future role of endocardial pacing and LBBAP in heart failure patients.