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To analyze the amount of free abdominal gas and ascites on computed tomography (CT) images relative to the location of a perforation.<br /><br /> We retrospectively included 172 consecutive patients (9379 = mf) with GIT perforation, who underwent abdominal surgery (ground truth for perforation location). The volume of free air and ascites were quantified on CT images by 4 radiologists and a semiautomated software. The relation of the perforation location (upper/lower GIT) and amount of free air and ascites was analyzed by the Mann-Whitney test. Furthermore, best volume cutoff for upper and lower GIT perforation, areas under the curve (AUC), and interreader volume agreement were assessed.<br /><br /> There was significantly more abdominal ascites with upper GIT perforation (333ml, range 5 to 2000ml) than with lower GIT perforation (100ml, range 5 to 2000ml, p = 0.022). The highest volume of free air was found with perforations of the stomach, descending colon and sigmoid colon. Significantly less free air was found with perforations of the small bowel and ascending colon compared to the aforementioned. An ascites volume &gt; 333ml was associated with an upper GIT perforation demonstrating an AUC of 0.63 ± 0.04.<br /><br /> Using a two-step process based on the volumes of free air and free fluid can help localizing the site of perforation to the upper, middle or lower GI tract.<br /><br />Using a two-step process based on the volumes of free air and free fluid can help localizing the site of perforation to the upper, middle or lower GI tract.<br /><br /> To evaluate the associations of DECT parameters with extracorporeal shock wave lithotripsy (ESWL) outcomes in pediatric patients.<br /><br /> A retrospective study of consecutive patients with calculi who underwent ESWL and DECT in our hospital was performed in 2011-2019. The primary outcome was DECT imaging's correlation with ESWL outcomes. The secondary outcome was to determine DECT parameters independently predicting ESWL outcomes, including stone-free (SF) and residual stone (RS) statuses.<br /><br /> The study included 207 patients. The mean CT attenuations at 140 kVp, 80 kVp, and 120 kVp and effective atomic number (Zeff) were significantly correlated with stone free (SF) and residual stone (RS) (P &lt; 0.05). Areas under the curves (AUCs) of CT attenuations at 120 kVp, 80 kVp, 140 kVp, and dual-energy index (DEI) were 0.784 (95% CI 0.672-0.897), 0.780 (95% CI 0.677-0.884), 0.766 (95% CI 0.658-0.885), and 0.709 (95% CI 0.578-0.840) (all P &lt; 0.05). With cutoffs of 882.5, 1330.5, 1042.5, and 0.103 for CT attenuations at 140 kVp, 80 kVp, 120 kVp, and DEI, respectively, sensitivities and specificities were 75.0% and 31.1%, 83.3% and 31.8%, 80.3% and 31.1%, and 58.3% and 44.7%, respectively.<br /><br /> Our results demonstrated that the parameters of DECT could be used to predict ESWL outcomes (especially the SF status) in children with urolithiasis. ESWL success can be accurately predicted by DECT, and it is hard to predict ESWL failure.<br /><br />Our results demonstrated that the parameters of DECT could be used to predict ESWL outcomes (especially the SF status) in children with urolithiasis. ESWL success can be accurately predicted by DECT, and it is hard to predict ESWL failure.The aim of study was to determine the influence of soluble and solid forms of Si on the growth of B. amyloliquefaciens. The experiment was conducted at two regimes under sterile conditions (without B. amyloliquefaciens) and infected conditions (with B. amyloliquefaciens). New formed silica gel, diatomite and monosilicic acid at 1 mM Si and 2 mM Si were used as source of Si. The concentration of monosilicic acid in the solution was measured on second and tenth days of experiment. The total carbon in the solution before and after centrifugation was determined on day 10 of the experiment. The experiment has demonstrated a significant positive effect (by 4.7-41.2%) on B. amyloliquefaciens growth in water system. The presence of B. amyloliquefaciens in Si-rich solution reduced the concentration of monosilicic acid in the solution up to 16.2%. About 13.5-30.7% of B. amyloliquefaciens can be attached to the Si-rich surface without formation of cell clusters. Si can be classified as a beneficial nutrient for B. amyloliquefaciens. The tested strain of Bacillus can form channels in silica gel. [https://www.selleckchem.com/products/AZD2281(Olaparib).html Olaparib] The presence of monosilicic acid resulted in the formation of an aligned positioning of cells in water-based solution. This study is the first to demonstrate the direct influence of active Si forms on bacteria growth. The research showed that monosilicic acid or Si-rich solid substances with high solubility on Si can be recommended to increase B. amyloliquefaciens growth in soil, water or reactors.This proof-of-principle study describes the development of a rapid and easy-to-use DNA microarray assay for the authentication of giant tiger prawns and whiteleg shrimp. Following DNA extraction and conventional end-point PCR of a 16S rDNA segment, the PCR products are hybridised to species-specific oligonucleotide probes on DNA microarrays located at the bottom of centrifuge tubes (ArrayTubes) and the resulting signal patterns are compared to those of reference specimens. A total of 21 species-specific probes were designed and signal patterns were recorded for 47 crustacean specimens belonging to 16 species of seven families. A hierarchical clustering of the signal patterns demonstrated the specificity of the DNA microarray for the two target species. The DNA microarray can easily be expanded to other important crustaceans. As the complete assay can be performed within half a day and does not require taxonomic expertise, it represents a rapid and simple alternative to tedious DNA barcoding and could be used by crustacean trading companies as well as food control authorities for authentication of crustacean commodities.Most of childhood hydrocephalus are originating during infancy. It is considered to be a complex disease since it is developed on the basis of heterogeneous pathophysiological mechanisms and different pathological conditions as well as during different age groups. Hence, it is of relevant importance to have a practical concept in mind, how to categorize hydrocephalus to surgically better approach this disease. The current review should offer further basis of discussion on a disease still most frequently seen in Pediatric Neurosurgery. Current literature on pathophysiology and classification of pediatric hydrocephalus has been reviewed to integrate the different published concepts of hydrocephalus for pediatric neurosurgeons. The current understanding of infant and childhood hydrocephalus pathophysiology is summarized. A simplified concept based on seven factors of CSF dynamics is elaborated and discussed in the context of recent discussions. The seven factors such as pulsatility, CSF production, major CSF pathways, minor CSF pathways, CSF absorption, venous outflow, and respiration may have different relevance and may also overlap for the individual hydrocephalic condition.