Zobrazení zdroje stránky Dissinggauthier5294

Taken together, these findings demonstrate the importance of investigating the reactivity of the oxytocin system and its relationship with social cognition. Our findings suggest that reduced emotional processing abilities may represent a pathological feature in a group of craniopharyngioma patients, indicating that this patient group might benefit from specific treatments within the social domain. © 2020 The Authors. Journal of Neuroendocrinology published by John Wiley &amp; Sons Ltd on behalf of British Society for Neuroendocrinology.BACKGROUND Hepatitis C virus (HCV) is a risk factor for porphyria cutanea tarda (PCT), a rare disease originating in the liver characterised by overproduction of porphyrins. Although hepatitis C infection is highly prevalent among patients with porphyria, only a minority of hepatitis C patients develop PCT. AIMS To explore the presence of porphyrin abnormalities in a cohort of asymptomatic hepatitis C-infected patients and the impact of anti-viral therapy. METHODS Eighty-four consecutive patients with HCV infection treated with direct-acting antivirals after 1 January 2018 were longitudinally evaluated for the presence of porphyrin abnormalities. Those patients with biochemical abnormalities at baseline were additionally evaluated at follow-up. Porphyrins in urine were screened by fluorometry and isomer separation was performed by liquid chromatography. RESULTS In five patients, all of them asymptomatic, porphyrin profile abnormalities were detected three presented significant increased urinary porphyrins with a typical PCT profile, and two showed normal levels of urinary porphyrins, but abnormal porphyria-like profiles. Urine evaluation after hepatitis C cure showed complete normalisation of the urinary porphyrins in all patients, confirming the biochemical cure of the disease. CONCLUSIONS We document the existence of rare cases of hepatitis C-infected patients with significant uroporphyrinuria in the absence of dermatological manifestations. Anti-viral therapy normalises the biochemical disorder, preventing patients from presenting PCT associated complications. © 2020 John Wiley &amp; Sons Ltd.Pancreatic cancer (PC) is a continuously high lethal disease, and the tumour microenvironment plays a pivotal role during PC progression. Herein, we focus on that the Nerve growth factor (NGF)/Tropomyosin-related kinase A (TrkA), in pancreatic stellate cells-pancreatic cancer cells (PSCs-PC cells) co-culture system, influences PC proliferation and invasion. The model of PC cells and PSCs was directly co-cultured in a no-touch manner, using the Transwell as the co-culture system. NGF and TrkA expression was measured in cultured system by real-time PCR, immunofluorescence, Western blotting analysis or ELISA. Small interfering RNA transfection was used to regulate the expression of TrkA in PC cells. The promotion of cancer invasion was investigated using Matrigel Transwell assay. In our study, NGF/TrkA is overexpressed in PSCs-PC cells co-culture system and promotes the invasion and proliferation of PC cells. And the epithelial-mesenchymal transition-related genes are influenced by si-TrkA. What's more, NGF/TrkA regulates the PC cell proliferation and invasion via activation of PI3K/AKT/GSK signalling. The present study demonstrated NGF/TrkA promoted the PC cell proliferation and invasion in the co-culture system by the activation of the PI3K/AKT/GSK signal cascade, providing a potential therapeutic target for PC patients. © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley &amp; Sons Ltd.OBJECTIVE This study investigates the effectiveness of self-nanoemulsifying drug delivery system (SNEDDS) in improving voriconazole transcorneal permeability. METHODS Voriconazole-SNEDDS was prepared with isopropyl myristate, PEG 400, Tween 80® and Span 80® and was subjected for physicochemical characterization after reconstitution with NaCl 0.9% (1/9; v/v). In-vitro antifungal activity was assessed and compared with the marketed formulation. In-vivo studies, namely ocular irritation test via modified Draize test and pharmacokinetic study, were investigated using rabbit as animal model. KEY FINDINGS Voriconazole-SNEDDS presented a droplet size of 21.353&nbsp;±&nbsp;0.065&nbsp;nm, a polydispersity index of 0.123&nbsp;±&nbsp;0.003, a pH of 7.205&nbsp;±&nbsp;0.006 and an osmolarity of 342.667&nbsp;±&nbsp;2.517&nbsp;mOsmol/l after reconstitution with NaCl 0.9%. Voriconazole-SNEDDS minimum inhibitory concentration (MIC90 ) was similar to the one of marketed formulation for Candida species while it was significantly lower (P&nbsp; less then &nbsp;0.001) for Aspergillus fumigatus. Draize test revealed that Voriconazole-SNEDDS was safe for ocular administration. [https://www.selleckchem.com/products/Cyclosporin-A(Cyclosporine-A).html selleck kinase inhibitor] Voriconazole maximum concentration (5.577&nbsp;±&nbsp;0.852&nbsp;µg/ml) from SNEDDS was higher than marketed formulation (Cmax &nbsp;=&nbsp;4.307&nbsp;±&nbsp;0.623&nbsp;µg/ml), and the Tmax was delayed to 2&nbsp;h. The area under the concentration-time curve value of Voriconazole-SNEDDS was improved by 2.419-fold. CONCLUSION Our results suggest that SNEDDS is a promising carrier for voriconazole ocular delivery and this encourages further clinical studies. © 2020 Royal Pharmaceutical Society.BACKGROUND Research is needed to identify how food insecurity affects maternal eating behavior and child feeding practices, factors that may pose intergenerational risks for obesity. OBJECTIVES This longitudinal study investigated whether maternal restrained eating mediated the association between household food insecurity and feeding practices. METHODS Participants included 277 WIC-eligible mothers (69% below the poverty line, 70% African American) and their toddlers (Mage = 20.11 months, SD = 5.50) participating in a childhood obesity prevention trial. Maternal reports of household food insecurity, restrained eating, and child feeding practices (restrictive and responsive) were collected at baseline, 6 and 12 months and analyzed using multilevel mediation. RESULTS Forty percent of mothers reported some degree of household food insecurity over 12 months. Within-person analyses showed that relative increases in household food insecurity were indirectly related to increases in restrictive and decreases in responsive child feeding practices, mediated through increases in mothers' own restrained eating.