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3% and 24.5%, respectively, with hookworms being the most detected parasites in dogs (8.3%) and Dipylidium caninum in cats (12.5%). Sex was the only variable that showed statistical differences in the canine population males being more often infected than female (p less then 0.05), while street access was associated with parasitic infections in cats (p less then 0.05). The results obtained in this study demonstrate the need to control parasitic infections in domiciled dogs and cats in different municipalities in the metropolitan region of the state of Rio de Janeiro, including residents in the northern, western and central areas.The utility of mitochondrial cytochrome oxidase subunit I (COX1) and 16S ribosomal DNA (16S-rDNA) sequence analyses as a complementary/alternative tool to classical taxonomy, for the identification of some of the most prevalent hard tick species from Portugal was evaluated using BOLD-ID (COX1 only), BLASTn and phylogenetic tree reconstruction based on multiple nucleotide sequence alignments. Both molecular markers proved suitable for identifying ticks to a species level, but specific aspects that limit their resolving power must be considered. Their accuracy of tick identification in all life stages and of the other tick species described in the South of Europe is required.Anaplasma marginale infection in cattle (n = 216) in the states of Uttar Pradesh and Uttarakhand, North India was screened by microscopy and nested-polymerase chain reaction (PCR). Two recombinant proteins viz. major surface protein (MSP) 5 and MSP2 of A. marginale were expressed in Escherichia coli and their potential in the detection of antibodies to Anaplasma species in the cattle was evaluated by immunoglobulin G-enzyme linked immunosorbent assay (IgG-ELISA). The MSP5 IgG ELISA results were compared with competitive (c) inhibition ELISA. Microscopy being the least sensitive diagnostic test detected 12.0% of animals positive for A. marginale infection while nested-PCR detected 87.9% of these animals as positive for A. marginale infection. The recombinant MSP5 antigen showed positive reactivity in 170/190 nested-PCR confirmed positive animals (sensitivity 89.5%) with specificity of 77.0%. In comparison, the recombinant MSP2 antigen showed lesser sensitivity and specificity of 79.0% and 69.2%, respectively. The cELISA was more sensitive and specific than IgG-ELISA. However, molecular detection by msp5 nested-PCR was highly sensitive and reliable for detection of carrier cattle for Anaplasma infection. The study indicated that a large cattle population (87.9%) was carrier for A. marginale infection in this region of the country.The present study explored the frequency of hair-sheep farms with gastrointestinal nematodes (GIN) resistant to albendazole sulfoxide (AS), ivermectin (IVM) and levamisole (LEV) in the Yucatán peninsula, México, using the faecal egg count reduction test (FECRT), and compared the frequency of farms diagnosed with resistance using three different formulae. The survey included farms from the states of Campeche (9) and Yucatán (14) (2016-2019). Collaborating farms had &gt;100 grazing ewes. Animals in the FECRT were &gt; 12 months old, received no anthelmintic for &gt;8 weeks and had ≥150 GIN eggs per gramme of faeces (EPG). Animals were distributed to respective groups untreated controls, AS (5 mg/kg BW), IVM (0.2 mg/kg BW), and LEV (7.5 mg/kg BW). Due to low EPG, some farms only included one or two AH groups. Second faecal samples were obtained on day 14 post-treatment to estimate the percentage reduction (%R) and 95% confidence interval (95%CI). Criteria to declare resistance were those proposed by the World Association cannot identify what proportion of the %R cannot be attributed to the AH. The untreated control group helped adjusting the %R calculation and seemed more adequate considering the propensity of hair-sheep to reduce their EPG on their own. .Small ruminant fasciolosis (SRF) is a snail - borne disease, transmitted by tropical freshwater snail, Lymnaea (Radix) natalensis in Nigeria. Fasciolosis in small ruminants is often neglected and information on the prevalence and economic losses are scarce. The aim of this study was to evaluate the prevalence of SRF and provide estimated annual economic losses associated with the disease. Meta-analysis revealed pooled prevalence of 2.1% (95%CI 0.0-5.8) in studies across Nigeria, while the distribution of L. natalensis was 13.2% (95%CI 11.6-14.1) among collected snails. Approximately 29.4% livestock owners disagree with veterinary inspectors on the liver condemnation of their slaughtered animals in the abattoir during meat inspection. No significant difference (P &gt; 0.05) was observed between SRF in sheep and goats. Mortality cost was estimated at US$ 55,671,620, while total liver condemnation was US$ 552,448. Direct losses were estimated at US$ 60,132,318 and indirect losses at US$ 17,807,706. The overall economic losses attributable to SRF in Nigeria was estimated at US$ 77,940,024 / annum (equivalent to 28.06 billion naira). Fasciolosis in small ruminant remains a major concern for Nigerian livestock industry. The present and future impact of the disease was further discussed. Considering the high economic burden of fasciolosis in small ruminants, improved disease surveillance and meat inspection, regular treatment and control campaigns are important to reduce infection rates and economic losses.South American opossums of the order Didelphimorphia are considered sentinels for zoonotic infections and environmental diseases, such as for Chagas disease caused by Trypanosoma cruzi. Nevertheless, there is a paucity of data regarding protozoan diseases such Toxoplasma gondii and Neospora caninum in Neotropical marsupials; despite these pathogens have been considered threats to some marsupial species. The aim of this study was to determine whether Didelphis albiventris and Philander frenatus opossums from southern Brazil had been previously exposed to T. cruzi, T. gondii or N. caninum. Opossum samples were obtained by live-trapping of free-ranging animals and collection at wildlife rehabilitation centers in Rio Grande do Sul (RS) state, Brazil. The detection of anti-T. cruzi and anti-T. gondii antibodies was performed by indirect hemagglutination and anti-N. caninum antibodies by competitive enzyme-linked immunosorbent assay. In total, samples were collected from 76 marsupials from nine municipalities in RS state, including 69 D. albiventris (white-eared opossum), and seven were P. frenatus (southern four-eyed opossum). For T. cruzi, 11% were seropositive, and for T. gondii 26% were seropositive. None of the marsupials sampled here were seropositive for N. caninum. Risk factor analysis showed that free-living animals were about five-fold more likely to be infected by T. gondii than were rescued animals. Our study showed the exposure of Neotropical marsupials (D. albiventris and P. frenatus) to protozoan pathogens T. cruzi and T. gondii, while no evidence of N. caninum exposure was found. The set of results presented here may have an effect on ecology and conservation of the studied species and may also indicate possible sentinels of these pathogen circulation.We describe the diagnostics surrounding the deaths of five working dogs over six-months to provide an enhanced clinical and diagnostic understanding of canine Chagas disease. Cases were five dogs with antibodies to Trypanosoma cruzi. Medical records were reviewed for diagnostic history. Testing was performed from samples collected before or immediately after death, including measurement of cardiac troponin I, histology, PCR and serology for Leishmania spp. and T. cruzi. Four dogs had a 2 to 7-year history of T. cruzi antibodies, while one positive dog had an unknown duration of exposure. Age at death ranged from 2 to 11 years and four dogs were actively working. The cardiac troponin I was elevated in all four dogs for which it was measured, although postmortem reference ranges are not established. Histopathologic diagnoses included mild to severe, chronic, lymphoplasmacytic to histiocytic myocarditis with variable fibrosis. Notably, only one dog had T. cruzi amastigotes observed in the heart histologically. T. cruzi DNA was detected in three other hearts. Although all five dogs tested antibody-positive for T. cruzi using three independent tests, all were also indirect fluorescent antibody-positive for Leishmania spp., interpreted as cross-reaction. [https://www.selleckchem.com/products/Perifosine.html Perifosine mouse] Chagas disease in dogs is a diagnostic challenge owing to cross-reactions and variable clinical, histologic and molecular presentations. The use and interpretation of multiple diagnostic strategies is useful in diagnosis. This study demonstrates techniques used to diagnose and characterize Chagas disease in an at-risk dog population.Platynosomum fastosum, which is distributed in tropical and subtropical regions, is the main parasite in the biliary system of domestic cats. Cats can be asymptomatic or show severe signs of cholangitis. Although the infection is reported throughout Brazil, only post-mortem studies are available from the Midwest region of the country. This cross-sectional study investigated the frequency of P. fastosum parasitism in domestic cats treated at the University Veterinary Hospital in Cuiabá city, Mato Grosso, Brazil, by three coproparasitological techniques (formalin-ether sedimentation, Faust, and Hoffmann), associating clinical, laboratory (haematological, biochemical) and ultrasonographic alterations. Based on the sample calculation, 171 cats were evaluated in the clinical and laboratory and a semi-structured epidemiological questionnaire was designed and used by the tutors. The prevalence observed was 26.90%, with no significant association with sex, reproductive status, age, hunting habit, access to the street, recent anthelmintic treatment, and the guardian's lack of knowledge regarding parasitism. Of the 171 cats evaluated, 55 (32.16%) showed clinical signs suggestive of cholangitis, with a significant association of this variable. Regarding the clinical and pathological variables, mild neutrophilia and elevated serum activity of alanine amino transferase and alkaline phosphatase was observed (but not significantly), while the mean values of eosinophils, leucocytes, total protein, albumin, and globulin were normal. The ultrasound findings were like those previously described. Thus, it was observed that P. fastosum occurs in moderate prevalence in cats from Cuiabá-MT, with a significant association with clinical signs and a regular agreement between the parasitological techniques employed.Sheep are intermediate hosts of the protozoon Toxoplasma gondii, a zoonotic parasite that that may cause abortion and reproductive losses in this species. Previous studies have shown that its prevalence is high in sheep herds in Brazil, but no information from the state of Espírito Santo was available. The aim of this study was to improve the epidemiological data in different regions of this state by analyzing occurrences of anti-T. gondii antibodies in young female sheep, before reproductive age, using IFAT (cutoff ≥64). In addition, variables relating to these animals and farm management were investigated for significance of associations. A total of 312 sheep, aged 3 to 12 months, on five farms in five municipalities, were sampled. Among these 312 animals, 135 (43.26%) were reactive, and 70 positive sheep (51.8%) presented high titers (more than three times the cutoff).Eight-month-old sheep presented the lowest occurrence and 7 and 11-month-old sheep, the highest. At least one animal was positive in each municipality, thus confirming the endemic status of toxoplasmosis in sheep in this region.