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•The analytical performance of the method and figures of merit including detection limits, precision, accuracy, and interferences when applied to surface water and groundwater matrices were investigated.A novel methodological approach was developed to quantified the volume of industrial waste desposal (IWD) site, combined with municipal waste materials (MWM), through the integration of a non-invasive, fast, and less expenssive RES2-D Electrical Resistivity Technique (ERT), using Wenner-Schlumberger electrode array geophysical method with Oasis Montaj software. Underground water bearing structures, and the eco-system are being contaminated through seepage of the plumes emanating from the mixtures of the industrial waste materials (IWM), made of moist cemented soil with municipal solid wastes (MSW) dumped at the site. The distribution of the contiminant hazardous plumes emanating from the waste materials' mixtures within the subsurface structural lithological layers was clearly map and delineated within the near-surface structures, using the triplicate technique to collect samples of the soil with the waste mixtures, and the water analysis for the presence of dissolved ions. [https://www.selleckchem.com/products/apr-246-prima-1met.html Eprenetapopt activator] The deployed method helped to monitor the seepage of the contaminant leachate plumes to the groundwater aquifer units via the ground surface, through the subsurface stratum lithological layers, and hence, estimation of the waste materials' volume was possibly approximated to be 312,000 m3. In summary, the novel method adopted are as presented below•The novel method is transferable, reproduce-able, and most importantly, it is unambiguous technique for the quantification of environmental, industrial and municipal waste materials.•It helps to map the distribution of the plumes emanating from the waste materials' mixtures within the subsurface structural lithological layers that was clearly delineated within the near-surface structures underlain the study site.•The procedure helped in the monitoring of leachate contaminants plumes seepages into the surface water bodies and the groundwater aquifer units, via the ground surface, through to the porous subsurface stratum lithological layers.4-hydroxyphenylpyruvate dioxygenase (HPD) is a key enzyme in the catabolism of tyrosine and therefore of great importance as a drug target to treat tyrosine-related inherited metabolic disorders (TIMD). Inhibition of this enzyme is therapeutically applied to prevent accumulation of toxic metabolites in TIMD patients. Nowadays an ex-herbicide, nitisinone, is used for this purpose and many more inhibitors are being explored and need to be tested. Here, we describe a colorimetric bacterial whole-cell screening system that allows quantifying the inhibitory effects of new human HPD inhibitors in a high-throughput and robust fashion. For this high-throughput screening (HTS) system we rely on the capability of recombinant E. coli that express human HPD, to generate a brown ochronotic pigment after the addition of tyrosine, whereafter this brown pigment can be quantified in a very specific and sensitive way by spectrophotometric analysis. Altogether, this robust and simple HTS screening system can be described as non-harmful, non-laborious and cost-effective with the aim to identify and evaluate novel therapeutic human HPD inhibitors for the treatment of TIMD.•This robust high-throughput screening system enables rapid identification and evaluation of potential inhibitors of human 4-hydroxyphenylpyruvate dioxygenase.•Simple and fast colorimetric quantification of the formation of ochronotic pigment.The Delphi technique is a suitable methodology for structuring group communication to answer current and prospective research questions within several rounds. The method is used in many disciplines and characterized by anonymity, iteration, controlled feedback, and statistical "group response" (Rowe &amp; Wright, 2001). This technical paper presents practical details and lessons learned from a two-round Delphi-based scenario study in which projections (Delphi statements, questions or hypotheses) were developed with findings from expert interviews and an expert workshop. This Delphi study provides answers to future-related questions for which other research methods are inappropriate. This is depicted as a five-step process, making it easy to follow and replicable, for example to help first-time Delphi-method researchers. In doing so, the authors aim to provide the community with valuable technical insights and guidance for studies applying the Delphi technique both to prospective questions and in other research settings.•Conducting a Delphi study can be a slow process with respect to receiving feedback from the panelists. Planning an appropriate period for distributing the questionnaire may produce a higher return rate. A sufficient time buffer should be incorporated into project planning.•Projections that create dissent among the panelists may provide valuable results.•Data analytics, software programs and online social networks can support researchers, save time and resources, and provide further insights in the process of conducting a Delphi study.A method was developed for creating single well-defined surface asperities using an imprint technique. The proposed method enables•Creation of well-defined micrometre high asperities•Creation of asperities which survived more than 35 million EHL contact cycles•Damage tracing thanks to the possibility to control the damage initiation sites.The technique is based on rolling a hard disc with indents against a soft disc for creating single surface asperities. The contact pressure causes plastic deformation forcing material into the indents to create the asperities. The height of the asperities can be controlled by adjusting the applied force. After initial reshaping during the run-in process, the asperities were strong enough to survive more than 35 million elastohydrodynamic lubrication cycles, which should be of great interest for the researchers who investigate rolling contact fatigue experimentally. The method could also aid the research on the run-in process by enabling tracing the development of specific surface defects.