Zobrazení zdroje stránky Aagesendamsgaard0322

lidation of fear extinction learning, thereby leading to reduced threat expectancies following reinstatement among women with PTSD. Future mechanistic research examining these and other biomarkers (e.g., brain-based biomarkers) is warranted.The amounts of inorganic ions (e.g. sodium, potassium, magnesium, calcium, chloride, and phosphate) in intrathecally administered oligonucleotide drug products need to be controlled in order to meet the final target formulation composition. During process development, these ions are measured to ensure the solution composition does not change during purification or drug product compounding and to ensure the final drug product targets are achieved. A quantitative method for ion analysis in antisense oligonucleotide (ASO) drugs was developed by mixed-mode anion exchange/cation exchange-hydrophilic interaction liquid chromatography (AEX/CEX-HILIC) with a charged aerosol detector (CAD). This thirteen-minute method measures monovalent and multivalent cations and anions simultaneously. LC separation conditions, CAD parameters, and sample preparation were judiciously optimized to ensure that the method is specific, accurate, precise, linear, robust, and rugged. With this LC-CAD approach, ion analysis for oligonucleotide drugs can be performed in most analytical labs to support drug development.A nanocomposite of molecularly imprinted polypyrrole on copper oxide (MIP@CuO) was introduced as a new coating for in-tube solid-phase microextraction (IT-SPME). The method coupled with HPLC-UV was successfully applied for analysis of carbamazepine (anticonvulsant and bipolar disorder medication) in biological samples. First, in order to increase the surface area and stability of the coating, copper oxide (CuO) nanosheets were synthesized on the inner surface of a copper tube using a chemical method. Then, molecularly imprinted polypyrrole coating (using carbamazepine as a template) was deposited on CuO by a facile in-situ electrodeposition method. According to the results, The MIP@CuO coating shows long life time, enhanced extraction efficiency, and good clean-up, for pre-concentration and determination of carbamazepine in biological samples. The synthesized adsorbent also showed high selectivity to carbamazepine compared to other drugs with similar structure. Important factors affecting the extraction efficiency of the analyte in the in-tube SPME method, such as salt concentration, extraction and desorption times, flowrates of the sample solution, and eluent, were optimized. Under optimal conditions, the method showed good linearity for carbamazepine in the range of 0.05-500 μg L-1, 0.10-500 μg L-1, and 0.10-500 μg L-1 in water, urine, and plasma samples, respectively, with coefficients of determination better than 0.996. The limits of detection were in the range of 0.01-0.05 μg L-1 in different matrices. The intra- and inter-assay precisions (RSD%, n = 3) were in the range of 6.7-8.1 % and 7.1-9.5 %, respectively.<br /><br /> The aim was to describe a population of older people in home health care based on what is probably a novel theoretical model, previously published, and to analyze longitudinal changes in different dimensions of nutritional status.<br /><br /> This explorative and longitudinal study examines nutritional status based on four domains in the novel theoretical model health and somatic disorders; cognitive, affective, and sensory function; physical function and capacity; and food and nutrition. Inclusion criteria were age ≥65 y and need of home health care for more than three months. A total of 69 men and women were enrolled in the study. Participants' nutritional status was studied at baseline and regularly during the following three years.<br /><br /> At baseline, 44% (n=27) reported one or more severe symptoms and 83% had polypharmacy (≥5 prescribed medications). The prevalence of malnutrition, sarcopenia, frailty, and dehydration at baseline were, respectively, 83% (n=35), 44% (n=24), 34% (n=18), and 45% (n=25). Participants that died during the 3-y follow-up (n=14) differed from survivors in the following aspects more reduced appetite, lower quality of life, worse cognitive function, lower physical activity, and less intake of dietary fiber and water. Dehydration at baseline was associated with lower function in several domains and with general decline over time.<br /><br /> Most participants had poor nutritional status. Dehydration and reduced appetite were important indicators of worsening nutritional and overall status and mortality.<br /><br />Most participants had poor nutritional status. Dehydration and reduced appetite were important indicators of worsening nutritional and overall status and mortality.Saccadic eye movements can drastically affect motion perception during saccades, the stationary surround is swept rapidly across the retina and contrast sensitivity is suppressed. However, after saccades, contrast sensitivity is enhanced for color and high-spatial frequency stimuli and reflexive tracking movements known as ocular following responses (OFR) are enhanced in response to large field motion. Additionally, OFR and postsaccadic enhancement of neural activity in primate motion processing areas are well correlated. It is not yet known how this postsaccadic enhancement arises. Therefore, we tested if the enhancement can be explained by changes in the balance of centre-surround antagonism in motion processing, where spatial summation is favoured at low contrasts and surround suppression is favoured at high contrasts. We found motion perception was selectively enhanced immediately after saccades for high spatial frequency stimuli, consistent with previously reported selective postsaccadic enhancement of contrast sensitivity for flashed high spatial frequency stimuli. The observed enhancement was also associated with changes in spatial summation and suppression, as well as contrast facilitation and inhibition, suggesting that motion processing is augmented to maximise visual perception immediately after saccades. The results highlight that spatial and contrast properties of underlying neural mechanisms for motion processing can be affected by an antecedent saccade for highly detailed stimuli and are in line with studies that show behavioural and neuronal enhancement of motion processing in non-human primates.Although the other-race effect (ORE; superior recognition of own- relative to other-race faces) is well established, the mechanisms underlying it are not well understood. We examined whether the ORE is attributable to differential use of shape and texture cues for own- vs. other-race faces. Shape cues are particularly important for detecting that an own-race face is unfamiliar, whereas texture cues are more important for recognizing familiar and newly learned own-race faces. We compared the influence of shape and texture cues on Caucasian participants' recognition of Caucasian and East Asian faces using two complementary approaches. In Experiment 1, participants studied veridical, shape-caricatured, or texture-caricatured faces and then were asked to recognize them in an old/new recognition task. In Experiment 2, all study faces were veridical and we independently removed the diagnosticity of shape (or texture) cues in the test phase by replacing original shape (or texture) with average shape (or texture). Despite an overall own-race advantage, participants' use of shape and texture cues was comparable for own- and other-race faces. These results suggest that the other-race effect is not attributable to qualitative differences in the use of shape and texture cues.Crossmodal correspondences are spontaneous associations of non-redundant information across different modalities. Infants and some non-human animals (i.e., chimpanzees, rhesus monkeys, and dogs) showed crossmodal correspondences like adult humans, suggesting a shared origin (at least among mammals) of such a phenomenon. Here we investigate visual-spatial crossmodal correspondences in a precocial avian species, i.e., the domestic chicken. Three-day-old chicks (n = 40) were presented with two (one in the left and one in the right hemispace of an arena) identical panels, either dyed black (low luminance) or white (high luminance). Chicks could circumnavigate either panel to obtain a food reward. Akin to humans, they preferentially chose the left side when presented with black panels and the right side when presented with white panels. The control group (n = 39), tested with grey panels, showed no spatial preference. In light of our results, we discuss crossmodal correspondences in terms of an early available mechanism widespread across different species.Brief periods of monocular deprivation significantly modify binocular visual processing. For example, patching one eye for a few hours alters the inter-ocular balance, with the previously patched eye becoming dominant once the patch is removed. However, the contribution of higher-level visual processing to this phenomenon is still unclear. Here, we compared changes in sensory eye dominance produced by three types of monocular manipulations in adult participants with normal binocular vision. One eye was covered for 150 min using either an opaque patch, a diffusing lens, or a prism that inverted the image. All three manipulations altered dominance duration and predominance during binocular rivalry (BR) in favour of the treated eye and the time courses of the changes were similar. These results indicate that modifications of luminance or contrast are not strictly necessary to drive shifts in eye dominance, as both were unaltered in the prism condition. Next, we found that shifts in eye dominance were dependent on attentional demands during the monocular treatment period, providing support for the role of attentional eye selection in modulating eye dominance. Finally, we found relatively rapid build-up of the ocular dominance shift after the onset of monocular treatment. Taken together, our results suggest that modifications to monocular input alter inter-ocular balance via selective attentional mechanisms that bias output towards the deprived eye. [https://www.selleckchem.com/products/abt-199.html find more] Eye-based attention may play an important role in conditions where normal input to one eye is disrupted, such as childhood amblyopia.Sensor network design is essential to efficiently integrate Structural Health Monitoring (SHM) systems in aerospace, automotive, and civil structures. This study describes an optimization model for piezoelectric (PZT) wafer placement on curved structures and closed sections. The proposed approach relied on the transformation of any complex/closed surface regardless of the shape of its cross-section into a flat plate and imposed a set of boundary conditions to account for the wave propagation characteristics. Because the structure was continuous and the wave could propagate in every direction, for simplicity and without sacrificing accuracy, our model assumed that a pair of PZT elements communicated information in the two shortest directions. Thus, the concept of having two paths for each PZT couple was introduced to tackle this multidirectional behavior. The plate was then discretized into a set of control points that represented the structure geometry. The PZT couples covered the control points along the line of sight and in the neighborhood of their direct and indirect paths.